The predominant female sex hormone estrogen impacts virtually all aspects of cardiovascular health. Estrogen receptors alpha and beta are classical estrogen receptors that have been extensively studied over the past several decades. The G protein-coupled estrogen receptor 1 (GPER, aka GPR30) was more recently identified and has since been implicated in numerous cardiovascular processes. This symposium will feature recent developments in GPER signaling mechanisms in the cardiovascular system, including novel findings, challenges, therapeutic applicability, and knowledge gaps. The presentations will incorporate paradigms in pharmacology, biochemistry, physiology, and pathophysiology with techniques spanning from the molecular to whole animal level.