This symposium will address some of the newer methods of unbiased, phosphoproteomic approaches to the analysis of physiological and pharmacological regulation of G-protein coupled receptor pathways. Several different G-protein coupled pathways will be discussed and compared. Data will be presented about how specific compartmentalization of pathways can be dissected using changes in phosphoproteomic profiles in the presence and absence of pathway selective drugs. There will also be a discussion of how phosphoproteomic analyses can be used in conjunction with cellular imaging techniques to analyze compartmentalized signaling.

Supported by the John V. Croker Fund